Glycoengineered yeast Pichia pastoris Expression System
Full-length IgG Yeast Surface Display
Schematic illustration of IgG yeast surface display
Full-length IgG library sorting by FACS
Advantages of Full-length IgG Yeast Surface Display
- In glycoengineered yeast P. pastoris, full-length IgG is displayed as close to native form in molecular structure, biophysical property, and biological function. However, ScFv in phage or yeast display requires format conversion into full-length IgG, which may lose activity.
- Comparing to the mammalian cell display, full-length IgG yeast surface display has many advantages, such as large library size, easy genetic manipulation, fast generation time, and low cost screening.
- FACS sorting of full-length IgG in yeast surface display system is based on both IgG antibody affinity and display level. It can eliminate the deviation caused by expression levels and distinguish clones with small affinity. However, phage display screen is usually adversely affected not only by affinity, but also by the expression level.
- FACS sorting technology can directly select high, medium, and low affinity clones at a time.