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Custom Antibodies for Your Targets

Speed up the development of monoclonal antibody and bispecific antibody with our glycoengineered yeast pichia pastoris expression system and full-length IgG yeast surface display technology. Genekine offers flexible and comprehensive services to produce custom antibodies.
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Custom Antibody Services

Humanization
  • CDR and SDR grafting.
  • Heavy chain CDR3 grafting.
  • Chain shuffling and guided selection.
Optimization
  • Binding affinity and specificity.
  • Expressibility.
  • Stability.
  • Species cross-reactivity.
Bispecific antibody
  • IgG-like bispecific antibody.
  • Common light chain isolation.

Accelerate success with our versatile IgG yeast surface display technology

Based on our proprietary platform of full-length IgG yeast surface display, Genekine provides comprehensive solutions for antibody humanization, affinity maturation, developability improvement, and bispecific antibody development. Clients will maximize their chances of identifying the best therapeutic or diagnostic antibody leads for the toughest targets.
Features
  • Large IgG antibody library
  • Yeast surface display of full-length IgG (Intact whole antibody)
  • Native IgG folding/pairing in display
  • IgG-like bispecific antibody
  • Multi-parameter FACS sorting
  • Excellent developability profiles

Schematic illustration of IgG yeast surface display

The yeast surface display of IgG antibody libraries can be generated by transformation of yeast P. pastoris with plasmids encoding for anchor protein, heavy chains and light chains. The full-length IgG antibody libraries are expressed and displayed on the surface of yeast cells. IgG-displaying yeast cells are incubated with the fluorophore-labeled antigen and fluorophore-labeled detection antibody for sorting by FACS. FACS sorting of IgG display libraries is based on both antibody affinity and display level.
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Contacts
Mail Address
142 Lowell Road Ste 17-216 Hudson, NH 03051