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Using Yeast Display of Full-length IgG and Bispecific Antibody to Boost the Success of Therapeutic Antibody

Using a unique technology that displays full-length IgG molecules on yeast cells, Genekine enhances the discovery and development of antibody drugs. Our clients can increase their chances of finding the best antibody leads for the most challenging targets, whether for therapeutic or diagnostic purposes.
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Custom Antibody Services

Humanization
  • CDR and SDR grafting.
  • Heavy chain CDR3 grafting.
  • Chain shuffling and guided selection.
Optimization
  • Binding affinity and specificity.
  • Expression level.
  • Stability.
  • Species cross-reactivity.
Bispecific antibody
  • IgG-like bispecific antibody.
  • Common light chain isolation.

Benefits of Genekine Technology

Genekine uses yeast Pichia pastoris expression system to create a proprietary technology of full-length IgG yeast surface display. Full-length IgG antibody and bispecific antibody are displayed as native forms in molecular structure and biophysical properties. The technique combines the high throughput of yeast display with mammalian-cell quality control. This innovative approach can be used to engineer monoclonal and bispecific antibodies with high affinity and improved developability profiles, which may not be possible using other display systems, such as ribosome display, phage display, traditional yeast display, and mammalian cell display.
Features
  • Yeast has quality control machinery similar to mammalian cells.
  • Yeast surface display of full-length IgG and bispecific antibodies.
  • Natural IgG folding in display.
  • Large full-length IgG libraries.
  • FACS real-time screening for both high affinity and expression.
  • Select antibodies with high affinity, diversity, and developability.
  • Isolate common light chains for bispecific antibody.

Schematic illustration of IgG yeast surface display

The yeast surface display of IgG antibody libraries can be generated by transformation of yeast P. pastoris with plasmids encoding for anchor protein, heavy chains and light chains. The full-length IgG antibody libraries are expressed and displayed on the surface of yeast cells. IgG-displaying yeast cells are incubated with the fluorophore-labeled antigen and fluorophore-labeled detection antibody for sorting by FACS. FACS sorting of IgG display libraries is based on both antibody affinity and display level.
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