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Antibody Assessment
Antibody production and characterization are important in research and biopharmaceutical development. Genekine can provide a comprehensive antibody production and analysis services to make the best understanding for interested antibody property.
Antibody characterization
- Concentration (UV280)
- Stability Tm (DSC) and Tagg (DLS)
- Purity analysis (SEC-HPLC, CE-SDS-NR)
- Charge variant analysis (icIEF)
- Affinity KD (ForteBio Octet)
Cell line development
- Cell clone screening
- Fed-batch evaluation
- PCB generation and stability study
- Media screening & ambr 15 mini bioreactor clone evaluation
Antibody production
- Mammalian cell transient expression
- Affinity purification
- 100ml pilot expression
- Scale up production (1L-10L)
In vitro pharmacology
- Growth inhibition (Luminescent cell viability assay)
- Growth inhibition (Cell Counting Kit-8 (CCK-8) assay)
- Assay in cell response, or intracellular signaling pathway
- Neutralization assay by reporter gene activity
- Cell proliferation (CellTiter-Glo, CFSE)
- Cytokine release (ELISA/Luminex), CRS assess
- CDC, ADCC, ADCP assay,
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